Performance comparison of three trypsin columns used in liquid chromatography

Recent advances in high-performance liquid affinity chromatography columns.

High-performance liquid affinity chromatography (h.p.1.a.c.) is the term applied to the application of h.p.1.c. to affinity chromatography (Ohlson et al., 1978). Until recently affinity chromatography supports were unsuitable for use in high-performace applications. It is only in the last 8 years that these problems have been overcome, resulting in a second generation of affinity systems. The a...

Microfabricated pillar array columns for liquid chromatography

High-Performance Liquid Chromatography Determination of Methotrexate in Plasma

This article describes a simple and fast high-performance liquid chromatography method for the determination of methotrexate (MTX) in plasma. Samples were collocted from children receiving high-dose MTX at shafa Hospital (Ahvaz University of Medical sciences, Ahvaz, iran) at various times after the end of eachinfusion. Plasma was deproteinized with trichloroacetic acid and the supernatant was i...

High-Performance Liquid Chromatography Determination of Methotrexate in Plasma

This article describes a simple and fast high-performance liquid chromatography method for the determination of methotrexate (MTX) in plasma. Samples were collocted from children receiving high-dose MTX at shafa Hospital (Ahvaz University of Medical sciences, Ahvaz, iran) at various times after the end of eachinfusion. Plasma was deproteinized with trichloroacetic acid and the supernatant was i...

High Performance Liquid Chromatography Determination of Sertraline in Human

       An accurate and sensitive reversed-phase high-performance liquid chromatograph-ic method for determination of sertraline in human serum is described using 4-chloro-7-nitrobenzofurazan as pre-column derivatization agent. The drug and an internal standard (azithrimycin) were extracted from serum using a mixture of diethyl ether-chloroform and subjected to the pre-column derivatization with...